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Related Experiment Videos

Slit and robo: expression patterns in lung development.

Mark A Anselmo1, Sussie Dalvin, Parthak Prodhan

  • 1Pediatric Pulmonary Unit, Massachusetts General Hospital for Children, Harvard Medical School, Jackson 14, GRJ 1416, 55 Fruit St, Boston, MA 02114, USA.

Gene Expression Patterns : GEP
|March 1, 2003
PubMed
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Slit and Robo proteins are expressed in developing mouse lungs, suggesting they play a role in pulmonary bronchiolar development. This study details their expression patterns during embryonic lung development.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Slit ligands and their Roundabout (Robo) receptors are known axonal guidance cues.
  • Previous indirect evidence suggested a role for Slit/Robo in lung development.

Purpose of the Study:

  • To investigate the expression patterns of Slit-2, Slit-3, Robo-1, and Robo-2 during embryonic murine lung development.
  • To determine the spatial and temporal distribution of these molecules within the developing lung.

Main Methods:

  • Immunohistochemistry was used to detect the expression of Slit-2, Slit-3, Robo-1, and Robo-2 proteins.
  • Expression patterns were analyzed in embryonic murine lung tissue at different developmental stages.

Main Results:

Related Experiment Videos

  • Slit-2 and Slit-3 were found to be developmentally regulated in embryonic murine lungs.
  • Both Slit-2 and Slit-3 were expressed by the pulmonary mesenchyme and airway epithelium.
  • Robo-1 and Robo-2 were also expressed in the developing mesenchyme and airway epithelium, with expression localizing to the airway epithelium as development progressed.

Conclusions:

  • Slit-2, Slit-3, Robo-1, and Robo-2 are expressed in temporo-spatially adjacent domains within the developing lung.
  • These findings suggest interactive roles for the Slit/Robo signaling pathway in pulmonary bronchiolar development.