HPLC for in-process control in the production of sultamicillin

A high-performance liquid chromatographic assay coupled with UV detection (215 nm) was developed for the determination of sultamicillin and its synthesis precursors. The separation of the analytes was performed on a Kromasil C(18) column (15 cm x 4.6 mm i.d., 5 microm) at 20 degrees C. The mobile phase (25 mM phosphate buffer, pH 7.0 and acetonitrile 48%) was pumped at a flow rate of 1.0 ml min(-1). This method is sensitive (limits of detection ranged between 0.4 and 1.2 mg l(-1)) and selective for the determination of sultamicillin and could be used for monitoring different synthetic routes.