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Related Experiment Videos

A rapid solid-phase fluorimetric assay for measuring bacterial adherence, using DNA-binding stains.

J A Bosch1, E C I Veerman, M Turkenburg

  • 1Department of Dental Basic Sciences, Section Oral Biochemistry, Vrije Universiteit, Academic Centre for Dentistry Amsterdam (ACTA), Van der Boechorststraat 7 1081 BT, Amsterdam, The Netherlands.

Journal of Microbiological Methods
|March 1, 2003
PubMed
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This study validates a rapid fluorescent method for quantifying bacterial adherence to saliva-coated surfaces. The assay is reliable and broadly applicable for studying microbial interactions in research.

Area of Science:

  • Microbiology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Bacterial adherence to host surfaces is crucial for colonization and infection.
  • Quantifying bacterial adherence often requires complex or hazardous methods.
  • Salivary components play a significant role in mediating bacterial interactions.

Purpose of the Study:

  • To validate a novel, rapid, single-step microtiter plate assay for quantifying bacterial adherence.
  • To assess the reliability and applicability of the assay for various bacterial species, including Helicobacter pylori and viridans streptococci.
  • To evaluate the temporal stability of saliva-mediated bacterial adherence.

Main Methods:

  • Developed a method using fluorescent DNA-binding probes for labeling microorganisms.

Related Experiment Videos

  • Tested bacterial binding to saliva-coated microtiter plates.
  • Assessed short-term and long-term temporal stability of adherence in a healthy population (N=30).

    • Main Results:

    • The assay demonstrated excellent reliability with within-assay variability coefficients from 4.9% to 11%.
    • Quantification ranged from approximately 5 x 10(4) to 1 x 10(7) cells.
    • Confirmed the method's applicability to diverse bacteria, including Helicobacter pylori and viridans streptococci.

    Conclusions:

    • The validated method offers a rapid, sensitive, and reliable approach for quantifying bacterial adherence.
    • This technique eliminates the need for radioactive materials or specific antibodies, simplifying quantification.
    • The assay is broadly applicable to various microbial species for basic and clinical research.