Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple micro-growth assay for enumerating bacteria.

Jeffrey D Brewster1

  • 1United States Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Philadelphia, PA 19038, USA. jbrewster@arserrc.gov

Journal of Microbiological Methods
|March 1, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enrichment, Amplification, and Sequence-Based Typing of Salmonella enterica and Other Foodborne Pathogens.

Journal of food protection·2017
Same author

Short communication: Improved method for centrifugal recovery of bacteria from raw milk applied to sensitive real-time quantitative PCR detection of Salmonella spp.

Journal of dairy science·2016
Same author

Antibody Microarray for E. coli O157:H7 and Shiga Toxin in Microtiter Plates.

Sensors (Basel, Switzerland)·2015
Same author

Two methods for increased specificity and sensitivity in loop-mediated isothermal amplification.

Molecules (Basel, Switzerland)·2015
Same author

DNA extraction protocol for rapid PCR detection of pathogenic bacteria.

Analytical biochemistry·2013
Same author

An antibody microarray, in multiwell plate format, for multiplex screening of foodborne pathogenic bacteria and biomolecules.

Analytical and bioanalytical chemistry·2008

A new method accurately measures bacteria concentration in liquids by monitoring growth turbidity. This simple assay overcomes condensation issues for rapid, reliable bacterial quantification.

Area of Science:

  • Microbiology
  • Biotechnology

Background:

  • Accurate bacterial quantification is crucial for various applications.
  • Existing methods can be time-consuming or require specialized equipment.
  • Nonspecific bacterial detection in liquid samples presents challenges.

Purpose of the Study:

  • To develop a simple, rapid, and accurate method for determining bacteria concentrations in liquid samples.
  • To overcome technical challenges in real-time turbidity measurements in microwell plates.

Main Methods:

  • A novel assay based on time to reach threshold turbidity was developed.
  • Samples were incubated in liquid media within a 96-well microwell plate.
  • Turbidity was monitored in real-time using a temperature-controlled plate reader.
  • A surface-active agent was used to prevent condensation on the microwell plate cover.

Related Experiment Videos

Main Results:

  • The assay demonstrated a relative error of approximately 20% for Salmonella and E. coli.
  • Accurate quantification was achieved for bacterial concentrations ranging from 10 to 10(6) cells/ml.
  • Assay setup and data processing were efficient, requiring 10 min and 20 min, respectively.
  • Bacterial growth times varied from 4 to 16 hours depending on initial concentration.

Conclusions:

  • The developed method provides a simple and effective approach for nonspecific bacteria determination.
  • The use of a surface-active agent successfully addressed condensation issues, enabling reliable turbidity measurements.
  • This assay offers a practical solution for rapid bacterial quantification in diverse liquid samples.