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The type B leukemogenic virus truncated superantigen is dispensable for T-cell lymphomagenesis
Farah Mustafa1, Sanchita Bhadra, Dennis Johnston
1Section of Molecular Genetics and Microbiology and Institute for Cellular and Molecular Biology, the University of Texas at Austin, Austin, Texas 78712, USA.
Abstract:
Type B leukemogenic virus (TBLV) is a variant of mouse mammary tumor virus (MMTV) that causes T-cell lymphomas in mice. We have constructed a TBLV-MMTV hybrid, pHYB-TBLV, in which 756 bp of the C3H MMTV long terminal repeat (LTR) was replaced with 438 bp of the TBLV LTR. Intraperitoneal injection of pHYB-TBLV transfectants consistently resulted in T-cell lymphomas in 50% of injected weanling BALB/c mice with an average latency period of 5.7 (+/- 1.5) months. Transfectants of pHYB-TBLV containing a double-frameshift mutation in the truncated superantigen gene (sag) induced T-cell lymphomas with similar incidences, latency periods, and phenotypes, suggesting that cis-acting elements in the TBLV LTR determine disease specificity.