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Related Experiment Video

Updated: Jul 11, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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Published on: June 23, 2022

Activation of lysosomal function during dendritic cell maturation.

E Sergio Trombetta¹, Melanie Ebersold, Wendy Garrett

¹Department of Cell Biology and Department of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520-8002, USA.

Science (New York, N.Y.)

|March 1, 2003

Summary

Dendritic cells (DCs) enhance antigen presentation during maturation by activating lysosomal function. This boosts peptide-MHC class II complex formation, crucial for T cell stimulation.

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Area of Science:

Immunology
Cell Biology

Background:

Dendritic cells (DCs) differentiate from immature antigen-capturing cells to mature T cell-stimulating cells upon stimulation.
During maturation, DCs enhance their ability to form peptide-major histocompatibility complex (MHC) class II complexes.

Purpose of the Study:

To elucidate the mechanism behind the enhanced peptide-MHC class II complex formation during dendritic cell maturation.

Main Methods:

Investigated the role of lysosomal function and vacuolar proton pump activity in dendritic cell maturation.
Assessed antigen degradation and peptide loading efficiency in immature versus mature DCs.

Main Results:

Immature DCs exhibited slow antigen degradation and inefficient peptide loading.
DC maturation activated the vacuolar proton pump, increasing lysosomal acidification and proteolysis.
This enhanced lysosomal activity facilitated efficient formation of peptide-MHC class II complexes.

Conclusions:

Lysosomal function is developmentally regulated in DCs for efficient antigen processing.
Activation of lysosomal pathways is a key mechanism driving DC maturation and T cell stimulation.