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Related Experiment Videos

[Ocular surface reconstruction by tissue engineering].

Shigeru Kinoshita1

  • 1Department of Ophthalmology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokoji, Kawaramachi-dori, Kamigyo-ku, Kyoto 602-0841, Japan.

Nippon Ganka Gakkai Zasshi
|March 4, 2003
PubMed
Summary
This summary is machine-generated.

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Tissue engineering offers new ways to reconstruct the ocular surface using stem cells. This study explores cultivated allogeneic corneal and autologous oral mucosal epithelial cells for severe eye disorders, showing promise for ocular surface stability.

Area of Science:

  • Ophthalmology and Regenerative Medicine
  • Tissue Engineering and Stem Cell Therapy

Context:

  • Severe ocular surface disorders pose significant challenges for vision restoration.
  • Current treatments often fall short for bilaterally affected patients.
  • Tissue engineering with somatic stem cells presents a promising second-generation therapeutic approach.

Purpose:

  • To investigate the efficacy of transplanted cultivated mucosal epithelia for ocular surface reconstruction.
  • To evaluate allogeneic corneal epithelial stem cells and autologous oral mucosal epithelial cells.
  • To identify potential stem cell markers for corneal epithelial stem cells.

Summary:

  • Transplantation of cultivated allogeneic human corneal epithelial sheets demonstrated effectiveness in stabilizing the ocular surface for conditions like Stevens-Johnson syndrome and chemical injuries, though some cases experienced rejection or infection.

Related Experiment Videos

  • Autologous cultivated oral mucosal epithelial sheets showed similar histology and expressed keratin 3, surviving transplantation and restoring corneal transparency in rabbit models, suggesting their potential as corneal epithelium substitutes.
  • Cultivated human corneal endothelial cell sheets were successfully xenografted in rabbits, maintaining high cell density and function.
  • Novel techniques identified candidate stem cell markers for corneal epithelial stem cells.

    • Impact:

    • Provides evidence for the potential of cultivated mucosal epithelial cell transplantation in treating severe ocular surface diseases.
    • Highlights the promise of autologous oral mucosal epithelial cells as a viable alternative for corneal epithelium regeneration.
    • Advances the understanding of corneal epithelial stem cell markers, crucial for future therapeutic development.