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Capture and detection of a quencher labeled oligonucleotide by poly(phenylene ethynylene) particles

Joong Ho Moon1, Robert Deans, Elizabeth Krueger

  • 1Nomadics, Inc., 61 Rogers Street, Cambridge, MA 02142, USA.

Chemical Communications (Cambridge, England)
|March 4, 2003
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...