Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cutaneous wound analysis using hyperspectral imaging.

S A Shah1, N Bachrach, S J Spear

  • 1TissueInformatics, Pittsburgh, PA, USA.

Biotechniques
|March 5, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent evidence-based developments in the treatment of DID.

Frontiers in psychiatry·2025
Same author

PROBING THE INTERPLAY OF PERFUSION AND PERMEABILITY IN BLADDER INFLAMMATION.

Continence (Amsterdam, Netherlands)·2025
Same author

[A multi-centre ROM study of the Guideline-Informed Treatment for Personality Disorders].

Tijdschrift voor psychiatrie·2023
Same author

Resolution of Virtual Depth Sectioning from Four-Dimensional Scanning Transmission Electron Microscopy.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2023
Same author

[How evidence-based is the guideline-informed treatment for personality disorders (GIT-PD)?]

Tijdschrift voor psychiatrie·2022
Same author

Factors limiting quantitative phase retrieval in atomic-resolution differential phase contrast scanning transmission electron microscopy using a segmented detector.

Ultramicroscopy·2022
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

Hyperspectral imaging offers a high-throughput method for wound identification. This technique accurately estimates wound dimensions, comparable to traditional bright-field microscopy and pathologist evaluation.

Area of Science:

  • Biomedical Imaging
  • Dermatology
  • Wound Healing Research

Background:

  • Accurate wound assessment is crucial for effective treatment and healing monitoring.
  • Current methods like bright-field microscopy can be time-consuming and subjective.
  • Exploring advanced imaging techniques can improve efficiency and objectivity in wound analysis.

Purpose of the Study:

  • To evaluate hyperspectral imaging (HSI) as a non-invasive, high-throughput method for cutaneous wound identification.
  • To compare HSI-based wound area quantification with traditional bright-field microscopy and pathologist assessment.
  • To investigate the potential of HSI in differentiating between healthy and injured dermal tissue.

Main Methods:

  • Correlative analysis of full-thickness cutaneous wounds in a porcine model using bright-field and hyperspectral imaging.

Related Experiment Videos

  • Acquisition of distinct spectral signatures for various cutaneous tissue types (epidermis, injured dermis, normal dermis).
  • Development of an algorithm for objective comparison between HSI-derived wound regions and manual pathologist tracings.

    • Main Results:

    • HSI successfully identified unique spectral signatures for different tissue types within cutaneous wounds.
    • A pseudo-color pattern emerged, consistently defining the central wound region by its spectral signature.
    • No significant difference was found in wound dimensions measured by HSI compared to pathologist evaluations using bright-field microscopy.

    Conclusions:

    • Hyperspectral imaging demonstrates efficacy as an alternative method for wound identification and estimation.
    • HSI provides a high-throughput approach that approximates the accuracy of standard bright-field imaging and expert pathologist evaluation.
    • This technology holds promise for objective, efficient wound assessment in research and clinical settings.