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(2-Naphthoxy)acetyl chloride, a simple fluorescent reagent
Tsai-Hui Duh1, Hsin-Lung Wu, Hwang-Shang Kou
1School of Chemistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Journal of Chromatography. A
|March 5, 2003
Summary
A new fluorescent reagent, (2-naphthoxy)acetyl chloride, enables sensitive detection of amino compounds. This chemical derivatization method is effective for analyzing pharmaceuticals like amantadine and memantine using HPLC.
Area of Science:
- Analytical Chemistry
- Organic Chemistry
- Chromatography
Background:
- Development of sensitive fluorescent reagents is crucial for analytical derivatization in chromatography.
- The naphthalene system with an auxochrome offers favorable spectrophotometric properties for detection.
Purpose of the Study:
- To introduce (2-naphthoxy)acetyl chloride as a novel fluorescent reagent for analytical derivatization.
- To evaluate its potential for sensitive detection of analytes containing amino groups.
Main Methods:
- Synthesis of (2-naphthoxy)acetyl chloride from (2-naphthoxy)acetic acid.
- Derivatization of amantadine and memantine with the reagent.
- Analysis of the resulting naphthoxy derivatives using High-Performance Liquid Chromatography (HPLC) with fluorimetric detection.
Main Results:
- The reagent was successfully synthesized and used for derivatization.
- Naphthoxy derivatives of amantadine and memantine were analyzed at sub-micromolar levels.
- Optimal excitation and emission wavelengths were determined as 227 nm and 348 nm, respectively.
Conclusions:
- (2-naphthoxy)acetyl chloride is a promising fluorescent reagent for sensitive detection of amino-containing compounds.
- The method is applicable to pharmaceutical analysis and potentially to biological amines.
- Further applications in sensitive fluorimetric derivatization are anticipated.