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Updated: Sep 27, 2026

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
Published on: June 7, 2016
A frame shifted disulfide bridged analogue of angiotensin II
Boris Schmidt1, Christian Kühn, Dennis K Ehlert
1TU Darmstadt, Institut for Organic Chemistry, Petersenstr. 22, D-64287 Darmstadt, Germany. schimbo@oc.chemie.tu.darmstadt.de
Abstract:
N-(2-Mercaptoethyl)glycine [NMGly] was incorporated into the 3 and 5 positions of angiotensin II and oxidized to give the corresponding cyclized disulfide c[NMGly(3,5)]Ang II. The binding affinity to the angiotensin II receptor (AT(1)) of this conformationally constrained analogue, which is related to the potent Ang II agonist c[Hcy(3,5)]Ang II, was examined. The analogue had no affinity to the AT(1) receptor. Theoretical conformational analysis was performed to compare the conformational characteristics of model compounds of c[Hcy(3,5)]Ang II and the frame shifted analogue c[NMGly(3,5)]Ang II in an attempt to explain the lack of affinity.
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