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Related Experiment Videos

Increase of functional diversity by alternative splicing.

Evgenia V Kriventseva1, Ina Koch, Rolf Apweiler

  • 1European Bioinformatics Institute (EMBL-EBI), Hinxton Wellcome Trust Genome Campus, Hinxton, Cambridge, UK CB10 1SD.

Trends in Genetics : TIG
|March 5, 2003
PubMed
Summary

Alternative splicing in proteins often inserts or deletes entire domains, rather than disrupting them. This suggests positive selection has significantly shaped the evolution of alternative splicing.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Structural Biology

Background:

  • Alternative splicing is a key mechanism for generating protein diversity.
  • Understanding the evolutionary pressures on alternative splicing is crucial.

Purpose of the Study:

  • To analyze large-scale protein isoform data from alternative splicing.
  • To investigate the structural and functional consequences of alternative splicing events within protein domains.

Main Methods:

  • Large-scale analysis of protein isoforms.
  • Prediction of functional effects using 3D protein structures.
  • Assessment of alternative splicing event targeting.

Main Results:

  • Alternative splicing frequently inserts or deletes complete protein domains.

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  • Disruption of domains is less common; when it occurs, it often mimics domain removal.
  • Short splicing events within domains target functional residues more often than expected.

    • Conclusions:

    • Alternative splicing events are not random; they often preserve or functionally alter entire protein domains.
    • The observed patterns suggest strong positive selection has driven the evolution of alternative splicing.