Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enhanced pharmaceutical analysis by CE using dynamic surface coating system.

K D Altria1

  • 1GlaxoSmithKline Research and Development, Pharmaceutical Development Europe, Park Road, Ware, Herts SG12 0DP, UK. kda8029@gsk.com

Journal of Pharmaceutical and Biomedical Analysis
|March 5, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modeling the electrophoretic mobility of analytes in binary solvent electrolyte systems in capillary electrophoresis using an artificial neural network.

Die Pharmazie·2005
Same author

Overview of the status and applications of capillary electrophoresis to the analysis of small molecules.

Journal of chromatography. A·2004
Same author

High-speed microemulsion electrokinetic chromatography.

Electrophoresis·2001
Same author

Selective and quantitative analysis of 4-hydroxybenzoate preservatives by microemulsion electrokinetic chromatography.

Journal of chromatography. A·2001
Same author

Background theory and applications of microemulsion electrokinetic chromatography.

Journal of chromatography. A·2000
Same author

Application of microemulsion electrokinetic chromatography to the analysis of a wide range of pharmaceuticals and excipients.

Journal of chromatography. A·2000
Same journal

Analyzing the impact of ionizable lipid identity, purity, and stability on lipid nanoparticle performance.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Application of two-dimensional liquid chromatography as a complementary technique to circular dichroism spectroscopy and high-resolution mass spectrometry for the characterization of GalNAc-siRNA conjugates.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

The transfer of per- and polyfluoroalkyl substances (PFAS) from mother to child: Comparison between maternal and cord blood in an Italian cohort.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

UHPLC/Q-TOF-MS-based blood-component profiling and multi-omics analysis reveal potential protective mechanisms of Shenzhuo Formula against diabetic kidney disease.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Multi-center study of the recombinant cascade reagent (kinetic chromogenic assay) as an alternative method for bacterial endotoxin testing: Method validation, product suitability, and consistency evaluation with limulus amebocyte lysate.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Simultaneous enantioselective separation of 2-, 3- and 4-chloromethcathinones using supercritical fluid chromatography-tandem mass spectrometry and its application to human oral fluid samples.

Journal of pharmaceutical and biomedical analysis·2026
See all related articles

A new dynamic surface coating significantly improves capillary electrophoresis (CE) performance for pharmaceutical analysis. This method enhances migration time repeatability and peak shape for basic drugs, advancing routine CE implementation.

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Pharmaceutical Analysis

Background:

  • Capillary electrophoresis (CE) faces challenges in routine pharmaceutical analysis due to poor migration time repeatability.
  • Implementing CE for routine drug analysis is hindered by variability within injections and between capillaries.

Purpose of the Study:

  • To evaluate the effectiveness of a dynamic surface coating in improving the routine performance of capillary electrophoresis.
  • To assess the impact of the dynamic coating on migration time repeatability and peak shape for basic drugs in pharmaceutical analysis.

Main Methods:

  • Utilized a dynamic surface coating in capillary electrophoresis.
  • Investigated the electro-osmotic flow generated by the coating at low pH.
  • Compared analysis times, migration time repeatability, and peak characteristics for basic drugs with and without the coating.

Related Experiment Videos

Main Results:

  • The dynamic surface coating generated significant electro-osmotic flow at low pH, reducing analysis times for basic drugs.
  • Demonstrated improved repeatability of migration times within injection sequences and between capillaries.
  • Observed reduced peak tailing for basic drugs, leading to better peak shapes and enhanced peak area integration precision.

Conclusions:

  • The dynamic surface coating system represents a significant advancement for the routine implementation of capillary electrophoresis in pharmaceutical analysis.
  • The improved performance metrics, including enhanced repeatability and peak shape, make CE a more robust technique for drug analysis.