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Related Experiment Videos

Assessing gene expression variation in normal human tissues using GeneTag, a novel, global, sensitive profiling

Lily Y Wong1, Andrea Hafeman, Victoria L Boyd

  • 1Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA.

Journal of Biotechnology
|March 5, 2003
PubMed
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GeneTag, a novel gene expression profiling method, identifies expressed genes without prior sequence knowledge. This study highlights individual gene expression variations crucial for interpreting transcript profiling experiments.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Gene expression profiling is essential for understanding biological processes.
  • Current methods often require prior sequence knowledge.
  • Individual variations in gene expression can impact experimental outcomes.

Purpose of the Study:

  • To introduce GeneTag, a novel method for gene expression profiling.
  • To apply GeneTag for analyzing gene expression variation in human liver.
  • To identify tissue-specific genes across human organs.

Main Methods:

  • GeneTag enables visualization, quantification, and identification of expressed genes.
  • The method is independent of underlying sequence information.
  • Expression data is stored in a database for analysis using proprietary software.

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Main Results:

  • GeneTag was applied to determine gene expression variation in individual human liver samples.
  • Tissue-specific genes were identified by comparing expression patterns across human organs.
  • Differentially expressed genes were rapidly identified via a sequence database link.

Conclusions:

  • GeneTag provides a versatile tool for gene expression profiling across species and tissues.
  • Understanding individual gene expression variation is critical for designing and interpreting transcript profiling studies.
  • This method aids in the identification of novel and known genes and tissue-specific expression patterns.