Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in the structure, composition and function of sarcoplasmic-reticulum membrane during development.

M G Sarzala, M Pilarska, E Zubrzycka

    European Journal of Biochemistry
    |September 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The comet assay as a method for assessing dna damage in cryopreserved samples.

    Cryo letters·2024
    Same author

    A comparison of the quality of plain yogurt and its analog made from coconut flesh extract.

    Journal of dairy science·2023
    Same author

    The use of Ctrough for the therapeutic drug monitoring of olaparib in patients with ovarian cancer.

    European review for medical and pharmacological sciences·2023
    Same author

    Non-Kolbe electrolysis of <i>N</i>-protected-α-amino acids: a standardized method for the synthesis of <i>N</i>-protected (1-methoxyalkyl)amines.

    RSC advances·2022
    Same author

    Clinical Implications of the Coexistence of Anemia and Diabetes Mellitus in the Elderly Population.

    Journal of diabetes research·2021
    Same author

    Double superior vena cava and left brachiocephalic vein agenesis: a rare systemic vein anomaly and potential source of cardiac implantable electronic device and central venous catheter placement complications.

    Folia morphologica·2021

    Sarcoplasmic reticulum development in rabbits shows immature vesicles in newborns lacking Ca2+ pump protein and surface particles. These features mature with age, correlating with increased Ca2+ uptake and ATPase activity.

    Area of Science:

    • Muscle physiology
    • Developmental biology
    • Biochemistry

    Background:

    • Sarcoplasmic reticulum (SR) is crucial for muscle contraction, regulating intracellular calcium levels.
    • Understanding SR development is key to comprehending muscle maturation and function.

    Purpose of the Study:

    • To characterize and compare the structural, chemical, and functional properties of SR vesicles during rabbit muscle development.
    • To investigate the developmental changes in Ca2+-pump protein, ATPase activity, and lipid composition of SR.

    Main Methods:

    • Differential centrifugation and sucrose gradient purification of microsomal fractions from rabbit muscle at various developmental stages.
    • Negative staining electron microscopy to visualize vesicle surface morphology.
    • Biochemical assays to determine protein content (Ca2+-pump), Ca2+-dependent and Mg2+-dependent ATPase activities, and lipid composition.

    Related Experiment Videos

    Main Results:

    • Immature SR vesicles from fetal and newborn rabbits are smooth, lacking the 4-nm surface particles seen in adult muscle.
    • The Ca2+-pump protein (100,000-dalton) is low in early development and increases with age, paralleling Ca2+ uptake and Ca2+-dependent ATPase activity.
    • Mg2+-dependent ATPase activity is high in early development and decreases with age, while lipid content is higher in immature SR.

    Conclusions:

    • SR maturation involves the gradual acquisition of Ca2+-pump protein and surface particles, correlating with enhanced Ca2+ handling capabilities.
    • Developmental changes in SR composition, including protein and lipid content, are essential for functional maturation of muscle.
    • The study highlights significant differences in SR structure and function between immature and adult rabbit muscle.