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Soluble glucocorticoid-induced tumor necrosis factor receptor (sGITR) increased MMP-9 activity in murine macrophage
Hee-Sook Lee1, Hyun-Hee Shin, Byoung S Kwon
1Department of Biological Sciences and the Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea.
Abstract:
Glucocorticoid induced tumor necrosis factor receptor (GITR), a new TNFR family, increased production of matrix matalloproteinase (MMP-9) in murine macrophages. Murine macrophages produced a band of gelatinolytic activity at 100 kDa when stimulated for 18 h with soluble GITR. MMP-9 was identified by gelatin zymography and Western blot. Previous results demonstrated that murine macrophages express GITR and GITR ligand constitutively. Induction of MMP-9 was synergistic with co-treatment of INF-gamma. MMPs could play a critical role in progression and promotion of tissue injury after inflammation stimulated by GITR/ligand system.