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Enhanced production of pectinase by Bacillus sp. DT7 using solid state fermentation
Des Raj Kashyap1, Sanjeev Kumar Soni, Rupinder Tewari
1Department of Microbiology, Panjab University, Chandigarh, 160014, India.
Bioresource Technology
|March 6, 2003
Summary
Bacillus sp. DT7 efficiently produces alkaline and thermotolerant pectinase using solid-state fermentation. Optimal conditions involved wheat bran, polygalacturonic acid, and multivitamins, yielding 8050 U/g.
Area of Science:
- Biotechnology
- Enzyme Technology
- Microbial Fermentation
Background:
- Pectinase enzymes are crucial in various industrial applications.
- Solid-state fermentation (SSF) offers an efficient method for enzyme production.
- Optimizing SSF parameters is key to maximizing enzyme yields.
Purpose of the Study:
- To optimize conditions for alkaline and thermotolerant pectinase production by Bacillus sp. DT7 using SSF.
- To identify key factors influencing pectinase yield.
Main Methods:
- Solid-state fermentation using Bacillus sp. DT7.
- Optimization of substrate composition (wheat bran, polygalacturonic acid), moisture content, and vitamin supplementation (neurobion).
- Incubation at 37°C for 36 hours.
Main Results:
- Bacillus sp. DT7 demonstrated high production of alkaline and thermotolerant pectinase.
- Maximum enzyme yield of 8050 U/g dry substrate was achieved.
- Optimal conditions included wheat bran, 1% polygalacturonic acid, 27 µl/g neurobion, and 75% moisture.
Conclusions:
- Solid-state fermentation is a viable method for producing high levels of pectinase.
- Specific supplements and controlled conditions significantly enhance enzyme production.
- Bacillus sp. DT7 is a promising source for industrial pectinase.