Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aptamer structures: a preview into regulatory pathways?

Nicolas Piganeau1, Renée Schroeder

  • 1Institute of Microbiology and Genetics, Vienna Biocenter, Dr. Bohrgasse 9/4, A-1030 Vienna, Austria.

Chemistry & Biology
|March 6, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNA polymerase II-binding aptamers in human ACRO1 satellites disrupt transcription <i>in cis</i>.

Transcription·2020
Same author

iRAPs curb antisense transcription in E. coli.

Nucleic acids research·2019
Same author

An Allosteric Ribozyme Regulated by Doxycyline.

Angewandte Chemie (International ed. in English)·2018
Same author

Nascent RNA signaling to yeast RNA Pol II during transcription elongation.

PloS one·2018
Same author

Natural RNA Polymerase Aptamers Regulate Transcription in E. coli.

Molecular cell·2017
Same author

Temperature-dependent sRNA transcriptome of the Lyme disease spirochete.

BMC genomics·2017
Same journal

The Hedgehog Pathway Effector Smoothened Exhibits Signaling Competency in the Absence of Ciliary Accumulation.

Chemistry & biology·2017
Same journal

DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells.

Chemistry & biology·2015
Same journal

Differential Regulation of Specific Sphingolipids in Colon Cancer Cells during Staurosporine-Induced Apoptosis.

Chemistry & biology·2015
Same journal

Synthetic Peptides as cGMP-Independent Activators of cGMP-Dependent Protein Kinase Iα.

Chemistry & biology·2015
Same journal

Unraveling the B. pseudomallei Heptokinase WcbL: From Structure to Drug Discovery.

Chemistry & biology·2015
Same journal

Vitamin C as Cancer Destroyer, Investigating Sulfhydration, and the Variability in CFTR Interactome.

Chemistry & biology·2015
See all related articles

A novel RNA aptamer structure was discovered that binds streptomycin. This bipartite fold may represent a class of structures in natural RNAs that regulate gene expression by binding metabolites.

Area of Science:

  • Structural biology
  • Molecular biology
  • Biochemistry

Background:

  • Recent findings indicate metabolites directly influence messenger RNA (mRNA) translation.
  • RNA aptamers are crucial for understanding RNA function and interactions.

Purpose of the Study:

  • To elucidate the crystal structure of a streptomycin-binding RNA aptamer.
  • To investigate the structural basis for antibiotic recognition by RNA.

Main Methods:

  • X-ray crystallography was employed to determine the three-dimensional structure.
  • Structural analysis was performed to characterize the RNA fold and antibiotic interaction.

Main Results:

  • The streptomycin-binding RNA aptamer exhibits a unique bipartite fold.

Related Experiment Videos

  • This fold is capable of effectively clamping the streptomycin antibiotic.

    • Conclusions:

    • The discovered RNA aptamer structure represents a novel fold for antibiotic binding.
    • Similar RNA structures may exist in nature to bind metabolites and regulate mRNA translation.