Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Migration of cochlear lateral wall cells.

George Dunaway1, Yashanad Mhaskar, Gary Armour

  • 1Department of Pharmacology, Southern Illinois University School of Medicine, PO Box 10629, Springfield, IL 62794-9629, USA. gdunaway@siumed.edu

Hearing Research
|March 6, 2003
PubMed
Summary

Apoptosis and proliferation maintain cochlear lateral wall cells. Marginal cells, though rarely dividing, are replaced by underlying cells through migration, preserving stria vascularis function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Possibility of Macrolides Causing Hearing Loss in Children-What Did You Say?

JAMA otolaryngology-- head & neck surgery·2022
Same author

D-methionine administered as late as 36 hours post-noise exposure rescues from permanent threshold shift and dose-dependently increases serum antioxidant levels.

International journal of audiology·2022
Same author

Preloaded D-methionine protects from steady state and impulse noise-induced hearing loss and induces long-term cochlear and endogenous antioxidant effects.

PloS one·2021
Same author

Transient Receptor Potential Channels and Auditory Functions.

Antioxidants & redox signaling·2021
Same author

D-methionine immediate and continued rescue after noise exposure does not prevent temporary threshold shift but alters cochlear and serum antioxidant levels.

International journal of audiology·2021
Same author

Regulator of G protein signaling 17 represents a novel target for treating cisplatin induced hearing loss.

Scientific reports·2021

Area of Science:

  • Ototolaryngology
  • Cell Biology
  • Auditory Science

Background:

  • The cochlear lateral wall, including the stria vascularis and spiral ligament, is crucial for maintaining hearing function.
  • Understanding the cellular mechanisms of apoptosis and proliferation is vital for preserving cochlear structure and function.

Purpose of the Study:

  • To investigate the roles of apoptosis and proliferation in the maintenance of cochlear lateral wall cells.
  • To determine the origin and replacement mechanisms of strial marginal cells.

Main Methods:

  • Apoptosis was detected using Hoechst fluorescence stain and TUNEL assay.
  • Cell proliferation was assessed by 5-bromo-2'-deoxyuridine (BrdU) incorporation and proliferating cell nuclear antigen (PCNA) presence.
  • A BrdU pulse-chase study was conducted to track cell replacement.

Related Experiment Videos

Main Results:

  • Apoptosis incidence was higher in strial marginal cells compared to intermediate and basal cells, but similar to spiral ligament cells.
  • While marginal strial cell division was infrequent, proliferation was observed in other stria vascularis and spiral ligament cells.
  • BrdU labeling indicated that marginal cells were replaced by underlying cells within 24 hours post-injection.

Conclusions:

  • Marginal cell replacement in the stria vascularis likely originates from underlying cells.
  • Cell migration is a significant mechanism for maintaining the structural integrity and function of the stria vascularis.
  • These findings contribute to understanding cochlear homeostasis and potential therapeutic targets for hearing loss.