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Screening of gene-associated polymorphisms by use of in-gel competitive reassociation and EST (cDNA) array

Koshichi Gotoh1, Michio Oishi

  • 1Human Gene 2, Kazusa DNA Research Institute, 2-6-7, Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan. koshichi@kazusa.or.jp

Genome Research
|March 6, 2003
PubMed

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...