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Related Experiment Videos

Differentially expressed nucleolar TGF-beta1 target (DENTT) in mouse development.

Laurent L Ozbun1, Alfredo Martínez, Jerry Angdisen

  • 1National Cancer Institute, Cell and Cancer Biology Branch, Rockville, Maryland 20850, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|March 6, 2003
PubMed
Summary

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Differentially expressed nucleolar TGF-beta1 target (DENTT) is a nuclear protein involved in mouse embryogenesis. DENTT shows spatiotemporal expression patterns and influences transcriptional responses, suggesting novel roles for the TTSN superfamily.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Differentially expressed nucleolar TGF-beta1 target (DENTT) is a novel gene identified in human lung cancer cells.
  • DENTT belongs to the TSPY/TSPY-like/SET/NAP-1 (TTSN) protein superfamily.
  • Understanding DENTT's function in mammalian development is crucial.

Purpose of the Study:

  • To characterize mouse DENTT (Differentially expressed nucleolar TGF-beta1 target).
  • To investigate the spatiotemporal expression pattern of DENTT during mouse embryogenesis.
  • To explore DENTT's role in cellular differentiation and transcriptional regulation.

Main Methods:

  • Sequence analysis of mouse DENTT.
  • Subcellular localization studies using GFP-tagged DENTT in COS-7 cells.

Related Experiment Videos

  • Expression analysis via RT-PCR, Northern hybridization, and Western blot.
  • Immunohistochemical staining of embryonic tissues.
  • In vitro studies with F9 embryonal carcinoma cells and reporter gene assays.

    • Main Results:

    • Mouse DENTT encodes a 77.6 kDa nuclear protein with high homology to human DENTT.
    • DENTT localizes to the nucleolus and is expressed throughout mouse embryogenesis in a spatiotemporal manner.
    • Expression is observed in the heart, brain, spinal cord, dorsal root ganglia, and developing cartilage/osteoblasts.
    • DENTT expression rapidly decreases upon differentiation induction in F9 EC cells.
    • DENTT modulates transcription of specific reporter genes, upregulating 3TPLux and downregulating COL7A1Luc.

    Conclusions:

    • Mouse DENTT is a nucleolar protein with dynamic expression during embryogenesis.
    • DENTT plays a role in the differentiation of various cell types, including neurons and osteoblasts.
    • The findings suggest novel functions for the TTSN superfamily in embryonic development and transcriptional modulation.