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Published on: May 27, 2019
Extracellular acidic polysaccharide production by a two-membered bacterial coculture
Shinya Kurata1, Kazutaka Yamada, Kyoko Takatsu
1Kankyo Engineering Corporation, 1 Kimitsu, Kimitsu-shi, Chiba 299-1141, Japan.
Abstract:
A two-membered coculture of strains KYM-7 and KYM-8, identified as Cellulomonas cellulans and Agrobacterium tumefaciens, respectively, produced a large amount of an extracellular polysaccharide, designated APK-78, from starch. Each strain in pure culture produced only very little amount of polysaccharide from starch; the coexistence of the two strains from the early stage of cultivation was indispensable for a large amount of polysaccharide to be produced. The polysaccharide APK-78 was acidic and composed of glucose, galactose, succinic acid, and pyruvic acid with a molar ratio of 8.1:1.0:1.7:1.0, indicating that it is a succinoglycan type of polysaccharide.
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