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Published on: January 12, 2024
A novel cellulolytic, anaerobic, and thermophilic bacterium, Moorella sp. strain F21
Shuichi Karita1, Kengo Nakayama, Masakazu Goto
1Department of Sustainable Resource Science, Faculty of Bioresources, School of Medicine, Mie University, Tsu 514-8507, Japan. karita@bio.mie-u.ac.jp
Abstract:
A cellulolytic and thermophilic anaerobe was isolated from soil. This bacterium made a halo on a roll-tube culture containing Avicel. Analysis of the PCR-based 16S rRNA gene sequence showed that the bacterium was closely related to Moorella thermoacetica. Scanning electron microscopy showed the bacterium is a rod and has no protuberant structure on the surface of cells growing on cellulose, suggesting that this strain is a non-cellulosomal cellulolytic bacterium. Carboxymethyl cellulase and xylanase activities were detected in the culture broth. A major fermentation product from ball-milled cellulose was acetate. This strain has a potential to convert cellulosic biomass to acetate, directly.
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