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Updated: Sep 27, 2026
CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells
Published on: October 18, 2014
Dna damage in peripheral blood nuclear cells assessed by comet assay from individuals submitted to scintigraphic
F J S Dantas1, J C P de Mattos, M O Moraes
1Departamento de Biofísica e Biometria, IBRAG, UERJ, Avenida 28 de Setembro, 87, Fundos 4 Andar, CEP 20551-030, Rio de Janeiro, RJ, Brazil.
Abstract:
Stannous chloride (SnCl2) is employed as a reducing agent to obtain Technetium-99m-labelled radiophamaceuticals in nuclear medicine kits, being injected endovenously in humans. Toxic effects of these kits were not studied, thus making it important to evaluate their impact in humans. In this study, the toxic effects were evaluated from peripheral blood nuclear cells (PBNC) from patients who received radiopharmaceuticals obtained using such kits. The analyses included results performed by comet assay. DNA damage was visualized in PBNC samples collected within a time up to 2 hr, and 24 hr after radiopharmaceutical injection in the patients. Initially we observed an increase of comet signals, which subsequently were reduced to zero after 24 hr. The diminishing of comet amounts probably is associated with DNA repair of damaged cells or with the elimination by apoptosis of cells whose DNA are not repaired.
