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A 2-oxoglutarate-dependent dioxygenase is integrated in DIMBOA-biosynthesis
Monika Frey1, Karin Huber, Woong June Park
1Lehrstuhl für Genetik, Technische Universität München, 85350 Freising, Germany.
Phytochemistry
|March 7, 2003
Abstract:
Benzoxazinoids are secondary metabolites of grasses that function as natural pesticides. While many steps of DIMBOA biosynthesis have been elucidated, the mechanism of the introduction of OCH(3)-group at the C-7 position was unknown. Inhibitor experiments in Triticum aestivum and Zea mays suggest that a 2-oxoglutarate-dependent dioxygenase catalyses the hydroxylation reaction at C-7. Cloning and reverse genetics analysis have identified the Bx6 gene that encodes this enzyme. Bx6 is located in the Bx-gene cluster of maize.