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Transposition of the Drosophila hydei Minos transposon in the mouse germ line

Dubravka Drabek1, Laskaro Zagoraiou, Ton deWit

  • 1Department of Cell Biology and Genetics, Faculty of Medicine, Erasmus University Rotterdam, The Netherlands.

Genomics
|March 7, 2003
PubMed

Insights

The Minos transposon system shows promise for insertional mutagenesis in mice. This study demonstrates its effectiveness in the mouse germ line for genetic analysis.

Area of Science:

  • Genetics
  • Molecular Biology
  • Transposon Technology

Background:

  • Insertional mutagenesis is a powerful tool for functional genomics.
  • Tc1/mariner transposons are mobile genetic elements with potential applications in various organisms.
  • The Minos transposon, from the Tc1/mariner superfamily, has been explored for its transposition capabilities.

Purpose of the Study:

  • To evaluate the suitability of the Minos transposon system for insertional mutagenesis in the mouse germ line.
  • To assess the efficiency and integration patterns of Minos transposition in mice.
  • To determine the potential of Minos for functional genomic analysis in mammals.

Main Methods:

  • Generation of transgenic mouse lines expressing Minos transposase in oocytes.
  • Development of a mouse line carrying nonautonomous Minos transposons.
  • Analysis of transposition frequency and integration sites in the progeny.

Main Results:

  • A transposition frequency of 8.2% was observed in the progeny from mice carrying both transgenes.
  • Analysis of new integration sites revealed a high frequency of transpositions to different chromosomes.
  • The Minos system demonstrated successful transposition in the mouse germ line.

Conclusions:

  • The Minos transposon system is suitable for insertional mutagenesis in the mouse germ line.
  • Minos transposition offers an effective strategy for generating genetic variation in mice.
  • This system holds potential for advancing functional genomic studies in the mouse model.

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