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Updated: Sep 27, 2026
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
Published on: October 31, 2019
Cloning and expression of a putative cyclodextrin glucosyltransferase from the symbiotically competent cyanobacterium
Johanna Wouters1, Birgitta Bergman, Sven Janson
1Department of Biology and Environmental Science, Kalmar University, Barlastgatan 1, S-391 82, Kalmar, Sweden. johanna.wouters@hik.se
Abstract:
A polymerase chain reaction-based method was used to isolate a Nostoc sp. PCC 9229 cDNA from infected glands of Gunnera chilensis. The complete gene sequence was isolated from a genomic Nostoc sp. PCC 9229 library. Sequence analysis showed 84% amino acid similarity to a putative cyclodextrin glycosyltransferase from Nostoc sp. PCC 7120 and the gene was therefore termed cgt. Southern blot revealed that the cgt gene was present in symbiotically competent cyanobacteria. The cgt gene was expressed in free-living nitrogen-fixing cultures in light or in darkness when supplemented with fructose. This is the first expression analysis of a cgt gene from a cyanobacterium.
