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Development and application of caged calcium.

Graham C R Ellis-Davies1

  • 1Department of Pharmacology and Physiology, Drexel University College of Medicine, Philadelphia, Pennsylvania 19102, USA.

Methods in Enzymology
|March 8, 2003
PubMed
Summary
This summary is machine-generated.

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Caged calcium compounds like DM-nitrophen and NP-EGTA are now mature tools for cellular physiology. Their successful application in demanding experiments, particularly in neurobiology, highlights their quantitative use in various cell types.

Area of Science:

  • Biochemistry
  • Cellular Physiology
  • Neurobiology

Background:

  • Several caged calcium compounds have been developed since 1986.
  • Three key compounds (DM-nitrophen, NP-EGTA, nitr-5) are commercially available.
  • Each compound offers unique properties influencing experimental choices.

Purpose of the Study:

  • To review the development and application of caged calcium compounds.
  • To highlight the suitability of these tools for quantitative cellular studies.
  • To assess the maturity of caged calcium as a tool in neurobiology.

Main Methods:

  • Review of synthesized caged calcium compounds.
  • Evaluation of commercially available options (DM-nitrophen, NP-EGTA, nitr-5).
  • Assessment of quantitative application methods in various cell types.

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Main Results:

  • DM-nitrophen, NP-EGTA, and nitr-5 are established caged calcium tools.
  • Quantitative methods enable their use in demanding cellular experiments.
  • Successful application in linking presynaptic calcium to postsynaptic function.

Conclusions:

  • Caged calcium compounds are mature and versatile tools.
  • Their quantitative application is feasible in diverse cell types.
  • These compounds are valuable for cellular physiology and neurobiology research.