Related Experiment Videos
Partial molecular characterization of some kiwi fruit cultivars by RAPD markers
Poonam Shirkot1, D R Sharma, T Mohapatra
1Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan 173230, India.
Abstract:
Molecular variability among seven cultivars of A. deliciosa var. deliciosa was investigated through RAPD markers. Thirty four decamer primers were screened generating polymorphic patterns of amplified DNA for these cultivars. Twenty one selected primers gave clear and reporducible patterns. A total of 430 bands were produced and 29.37% of them were polymorphic. The patterns distinguished between the cultivars and their analysis established an approach to classification within A. deliciosa var. deliciosa based on RAPD markers. The dendrogram clearly differentiated male from female cultivars. While abbot and allison female cultivars were closely related, bruno and abbot female cultivars showed maximum dissimilarity.