Related Experiment Video
Updated: Sep 27, 2026
Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine
Published on: February 24, 2023
Effect of electrically mediated intratumor and intramuscular delivery of a plasmid encoding IFN alpha on visible B16
Loree C Heller1, Stephanie F Ingram, M Lee Lucas
1University of South Florida, Center for Molecular Delivery, Department of Surgery MDC16, 12901 Bruce B. Downs Blvd., Tampa, FL 33612, USA. lheller@hsc.usf.edu
Abstract:
Interferon alpha may be used as a single agent therapy for metastatic malignant melanoma or as an adjuvant to chemotherapy. Delivery of interferon alpha by gene therapy offers an alternative to recombinant protein therapy. Electrically mediated delivery enhances plasmid expression in a number of tissues, for instance skin, liver, muscle and tumors including melanomas. Here we compare the effect of delivery of a plasmid encoding mouse interferon alpha on growth of visible B16 mouse melanomas following electrically mediated delivery to muscle or directly to the tumor. Intratumoral delivery of interferon alpha plasmid not only slows melanoma growth, but induces complete, long term, regression. This effect was not observed after intramuscular delivery.
