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Updated: Aug 8, 2026
FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
[Detect maternal serum fetal DNA for prenatal diagnosis]
Yanyi Huang1, Xiaoman Chen, Shu Kong
1Department of Obstetrics and Gynecology, Second Municipal People's Hospital of Guangzhou Medical College, Guangzhou 510150, China.
Objective:
To detect maternal serum fetal DNA for prenatal diagnosis of fetal sex.
Methods:
Five to ten ml maternal serum was obtained from 24 pregnant women with 16 male fetuses and 8 female fetuses (including 4 carriers with X-linked diseases). The fetal sex was detected by analyzing the karyotype from amniotic fluid and villus. Serum DNA was extracted, nested polymerase chain reaction was employed the male fetal DNA.
Results:
The sensitivity of the first polymerase chain reaction was 62.5%. The sensitivity of the first and the second reaction was 100%. There was no false positive. Fetal sex of 4 X-linked disease carriers was diagnosed. The determinate rate was 100%.
Conclusion:
Using fetal DNA from maternal serum and nested polymerase chain reaction was a quick, simple and accurate method for prenatal diagnosis of the fetal sex.
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