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Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China
Guifu Peng1, Zhibing Wang, Shanshan Wang
1Department of Epidemiology, Medical Institute, Guangzhou Command PLA, Guangzhou 510507, China.
Objective:
To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region.
Methods:
The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed.
Results:
Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands.
Conclusion:
Nan Peng Lie Islands is tsutsugamushi disease nature foci.
