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ATP diphosphohydrolase in human platelets from patients with coronary arteries heart disease
E R Rossatto1, L B da Silva, G S Pereira
1Laboratório de Pesquisas Biomédicas, Serviço de Cardiologia, Hospital de Clínicas de Porto Alegre, RS, Brazil.
Insights
Platelet ATP diphosphohydrolase activity did not differ in patients with coronary artery disease. However, acetylsalicylic acid inhibited this enzyme, suggesting a link between ATP diphosphohydrolase and blood clot formation.
Area of Science:
- Biochemistry
- Cardiovascular Science
- Hematology
Background:
- Platelet ATP diphosphohydrolase may regulate ADP-dependent platelet aggregation.
- Platelet aggregation is crucial in coronary artery disease (CAD) pathogenesis.
- Understanding enzyme activity in CAD patients is important for thrombogenesis research.
Purpose of the Study:
- To determine ATP diphosphohydrolase activity in platelets from healthy individuals and patients with chronic or acute CAD.
- To investigate the in vitro effects of cardiovascular drugs on platelet ATP diphosphohydrolase activity.
Main Methods:
- Collected platelets from three groups: healthy controls, chronic CAD patients, and acute CAD patients.
- Assayed ATP diphosphohydrolase activity across groups.
- Tested the in vitro effect of acetylsalicylic acid (2.0 mM) on human platelet ATP hydrolysis.
Main Results:
- No significant differences in ATP diphosphohydrolase activity were found between healthy individuals and CAD patient groups.
- Control group exhibited a lower range of enzyme activity compared to patient groups.
- Acetylsalicylic acid inhibited platelet ATP hydrolysis by approximately 55% in vitro.
- Correlations observed between ADP hydrolysis and glucose levels (controls), and ATP hydrolysis and triglycerides (chronic CAD).
Conclusions:
- Platelet ATP diphosphohydrolase activity does not appear to differ significantly in patients with chronic or acute coronary artery disease.
- Acetylsalicylic acid demonstrates inhibitory effects on ATP diphosphohydrolase activity in vitro.
- Findings suggest a potential relationship between ATP diphosphohydrolase, its substrates/products, and thrombogenesis in cardiovascular disease.
Abstract:
ATP diphosphohydrolase is an enzyme described in platelets and may be related to the control of ADP-dependent platelet aggregation. Platelet aggregation in atherosclerotic coronary arteries, and the release of platelet-derived factors, play an important role in coronary artery disease syndromes. In this study, we determined the activity of ATP diphosphohydrolase in platelets from patients with chronic and acute coronary artery disease syndromes and healthy persons. The following groups were studied: healthy persons (group I), patients with chronic heart disease (group II) and acute heart disease (group III). Results did not demonstrate differences between the groups studied. The control group demonstrated a lower range of enzyme activity. The patients from groups II and III had ingested drugs with actions upon the cardiovascular system and the effect, in vitro, of these drugs upon the ATP diphosphohydrolase activity in human platelets was also investigated. The in vitro experiments demonstrated that 2.0 mM acetylsalicylic acid inhibited ATP hydrolysis by human platelets by approximately 55%. Significant correlation was observed between ADP hydrolysis and glucose blood levels in the control group and between ATP hydrolysis and triglycerides in the group II. These results contribute to our understanding of a possible relationship between ATP diphosphohydrolase and thrombogenesis.