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Streptomyces coelicolor A3(2) plasmid SCP2*: deductions from the complete sequence
Iris Haug1, Anke Weissenborn2, Dirk Brolle3
1Institut für Industrielle Genetik, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
Microbiology (Reading, England)
|March 8, 2003
Summary
The SCP2* plasmid from Streptomyces coelicolor A3(2) was fully sequenced, revealing 34 open reading frames and novel genes involved in replication, transfer, and stability, including site-specific recombinases and ATPases.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Plasmid SCP2* is a fertility factor in Streptomyces coelicolor A3(2).
- Previous studies identified replication, transfer, and stability regions within the plasmid.
Purpose of the Study:
- To perform complete sequencing of the SCP2* plasmid.
- To identify and characterize genes within the identified functional regions.
Main Methods:
- Whole-genome sequencing of Plasmid SCP2*
- Bioinformatic analysis of open reading frames (ORFs)
- Deletion analysis to narrow down functional regions
- Plasmid stability testing
Main Results:
- Sequencing revealed 31,317 bp with 34 ORFs, many novel.
- Identified stability genes mrpA, parA, and parB, with ParA similar to plasmid partition ATPases.
- Replication region narrowed to 1.6 kb containing repI and repII genes.
- Discovered two transposable elements: IS1648 and Tn5417.
Conclusions:
- The complete sequence of SCP2* provides insights into its genetic organization.
- Novel genes like mrpA, parA, and parB are crucial for plasmid stability.
- The identified genes and elements contribute to the plasmid's role as a fertility factor.