Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ion-extraction ladder sequencing from bead-based libraries.

Jose J Pastor1, Iain Lingard, Gurdip Bhalay

  • 1Departament de Química Orgànica, Universitat de Barcelona, Martí i Franquès, 1-11, 08028 Barcelona, Spain.

Journal of Combinatorial Chemistry
|March 11, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Caspofungin-Based Red-Emissive Probes for Fluorescent Imaging of Pathogenic Fungi.

JACS Au·2026
Same author

Structure-Property Relationships of Near-Infrared Cyanine Dyes: Chalcogen-Driven Singlet Oxygen Generation with High Fluorescence Efficiency.

ACS omega·2026
Same author

Author Correction: A fluorogenic, peptide-based probe for the detection of Cathepsin D in macrophages.

Communications chemistry·2026
Same author

Selective agonism of liver and gut FXR prevents cholestasis and intestinal atrophy in parenterally fed neonatal pigs.

Journal of lipid research·2025
Same author

Diagnostics at the frontline: using the Public Accounts Committee report to catalyse the UK's antimicrobial resistance diagnostics strategy.

The Lancet. Microbe·2025
Same author

Correction to "Target Engagement Assays in Early Drug Discovery".

Journal of medicinal chemistry·2025
Same journal

Editorial.

Journal of combinatorial chemistry·2016
Same journal

2000 Reviewers.

Journal of combinatorial chemistry·2016
Same journal

Editorial.

Journal of combinatorial chemistry·2016
Same journal

Editorial.

Journal of combinatorial chemistry·2016
Same journal

Editorial.

Journal of combinatorial chemistry·2016
Same journal

Editorial.

Journal of combinatorial chemistry·2016
See all related articles

This study introduces ion-extraction mass spectrometry for sequencing single beads. This method provides clear peptide identification, surpassing older library analysis techniques.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Proteomics

Background:

  • Traditional peptide sequencing methods face limitations in throughput and accuracy.
  • Bead-based assays are increasingly used but require robust analytical techniques for characterization.

Purpose of the Study:

  • To develop and validate a novel method for unambiguous sequencing of peptides immobilized on single beads.
  • To demonstrate the advantages of the new technique over existing library analysis approaches.

Main Methods:

  • Utilizing ion-extraction mass spectrometry to analyze mixtures of isotopically labeled compounds.
  • Applying the technique to analyze peptide sequences derived from single beads.

Main Results:

Related Experiment Videos

  • Achieved unambiguous sequencing of bead-based peptides.
  • Demonstrated superior performance compared to traditional library analysis.

    • Conclusions:

    • Ion-extraction mass spectrometry is a powerful tool for single-bead peptide sequencing.
    • This method offers significant advantages for high-throughput and accurate proteomic analysis.