Method for analysis of polymer-supported organic compounds using mass spectrometry direct insertion

Area of Science:

• Analytical Chemistry
• Organic Chemistry
• Polymer Chemistry

Background:

• Solid-phase synthesis is crucial for organic compound preparation.
• Analyzing compounds directly from solid supports presents analytical challenges.
• Existing methods for solid-supported compound analysis can be complex.

Purpose of the Study:

• To develop a simple and efficient mass spectrometry method for analyzing organic compounds supported on solid phases.
• To utilize the thermal instability of benzylic groups for compound cleavage.
• To demonstrate the applicability of the method for identifying various supported compounds.

Main Methods:

• Direct-insertion mass spectrometry with a quadrupole detector.
• Utilizing high temperatures within the mass spectrometer chamber for thermal cleavage of supported compounds.
• Analysis of fragmentation patterns and molecular ions for compound identification.

Main Results:

• A new, efficient method for analyzing solid-supported organic compounds was successfully developed.
• The thermal cleavage of benzylic groups in Merrifield resin enabled compound release.
• Identified various compounds, including bis-o-aminobenzamides, aromatic, and aliphatic compounds, with minimal interference from polymer degradation.

Conclusions:

• The developed mass spectrometry approach offers a straightforward and effective means for analyzing solid-supported organic compounds.
• The method is robust, with polymer degradation fragments not interfering with spectral interpretation.
• This technique simplifies the analysis of diverse compounds synthesized on solid supports.