Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Highly efficient, large volume flow electroporation.

Lin-Hong Li1, Rama Shivakumar, Stephanie Feller

  • 1MaxCyte, Inc., 9640 Medical Center Drive, Rockville, MD 20850, USA.

Technology in Cancer Research & Treatment
|March 11, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic maintenance of breast cancer cells and metastases through E-cadherin/YAP-dependent pyruvate carboxylase expression.

bioRxiv : the preprint server for biology·2026
Same author

3D "Emboli" Culture Models Epithelial Breast Cancer Cell Oxidative Mitochondrial Metabolism with Relevance for Lung Metastasis.

Cancer research communications·2026
Same author

A simple liquid 3D cell culture paradigm models oxidative mitochondrial metabolism of epithelial breast cancer cells with relevance for lung metastases.

bioRxiv : the preprint server for biology·2025
Same author

SOHO State of the Art Updates and Next Questions | The Current State of CAR T-Cell Therapy and Bispecific Antibodies in Mantle Cell Lymphoma.

Clinical lymphoma, myeloma & leukemia·2024
Same author

Paragonimiasis misdiagnosed as liver abscess: A case report.

World journal of clinical cases·2024
Same author

Taking a BiTE out of Lymphoma: Bispecific Antibodies in B-Cell Non-Hodgkin Lymphoma.

Cancers·2024
Same journal

Bridging Global Attention and Local Hierarchies: A Robust Hybrid Ensemble Framework With Multi-Perspective Explainability for Automated HER2-IHC Scoring.

Technology in cancer research & treatment·2026
Same journal

Research on Predicting the Efficacy of Immunotherapy for Non-small Cell Lung Cancer Based on CT Radiomics and Peripheral Blood Inflammatory Markers.

Technology in cancer research & treatment·2026
Same journal

Survival Prediction in Patients With Epidermal Growth Factor Receptor-Mutated Non-small Cell Lung Cancer With Bone Metastases Receiving Icotinib Therapy: A Retrospective Observational Study.

Technology in cancer research & treatment·2026
Same journal

Proteomics in Acute Myeloid Leukemia: Current Applications in Precision Medicine and Targeted Immunotherapy.

Technology in cancer research & treatment·2026
Same journal

EML4-ALK in Non-small Cell Lung Cancer: Molecular Mechanisms and Targeted Therapies.

Technology in cancer research & treatment·2026
Same journal

Developing and Validating a Risk Model for Severe Bone Marrow Suppression in Esophageal Cancer Treated with Radiotherapy or Chemoradiotherapy: A Retrospective Cohort Study.

Technology in cancer research & treatment·2026
See all related articles

This study introduces a scalable flow electroporation system for efficient large-volume cell transfection. The novel method achieves high cell viability and transfection efficiency, paving the way for clinical gene therapy and bioprocessing.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Genetic Engineering

Background:

  • Electroporation is a key technique for cell transfection and molecule loading.
  • Traditional static electroporation is limited to small volumes (<1 mL), hindering clinical and industrial applications.
  • There is a need for scalable electroporation methods for bioprocessing.

Purpose of the Study:

  • To develop and evaluate a scalable-volume flow electroporation system.
  • To assess the efficiency and viability of large-volume cell transfection using this system.
  • To demonstrate the in vivo efficacy of cells transfected via flow electroporation.

Main Methods:

  • Utilized a scalable-volume flow electroporation system for suspended (Jurkat) and adherent cells (10T1/2, Huh-7).

Related Experiment Videos

  • Measured macromolecule uptake (FITC-dextran) and plasmid transfection efficiency (eGFP) via flow cytometry.
  • Compared flow electroporation with static electroporation and evaluated transgene expression with EBNA1-OriP plasmids.
  • Assessed in vivo gene delivery efficacy using mIL12-transfected 10T1/2 cells in mice.

    • Main Results:

    • Achieved >90% electroloading efficiency for FITC-dextran and maintained >90% cell viability.
    • Demonstrated high flow electrotransfection efficiency (up to 75%) and cell viability (up to 90%) for volumes from 1.5 to 50 mL.
    • Observed no significant difference in transfection efficiency between flow and static methods.
    • Showcased a 500-1000 fold increase in transgene expression using EBNA1-OriP plasmids.
    • Confirmed functional in vivo gene delivery and therapeutic effect (anti-angiogenic activity) of mIL12-transfected cells.

    Conclusions:

    • The scalable flow electroporation system enables efficient large-volume cell transfection with high viability.
    • This technology offers a viable alternative to static electroporation for clinical gene therapy and bioprocessing.
    • Flow electrotransfected cells can serve as effective in vivo gene delivery vehicles.