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Homemade Site Directed Mutagenesis of Whole Plasmids
Published on: May 11, 2009
Kinetic and site-directed mutagenesis studies of prevotella intermedia acid phosphatase
Xiaochi Chen1, Toshihiro Ansai, Sailen Barik
1Department of Preventive Dentistry, Kyushu Dental College, Kitakyushu 803-85802 Japan.
Protein and Peptide Letters
|March 11, 2003
Abstract:
Site-directed mutagenesis was used to examine the roles of the conserved histidine, arginine and cysteine residues in acid phosphatase from Prevotella intermedia (PiACP). The replacement of histidine and arginine residues resulted in the elimination of the PiACP activity while the cysteine mutants retained activity. These results suggest that the histidine and arginine residues are essential for catalysis.

