Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Novel approaches to transdifferentiation.

Anne-Mari Håkelien1, Philippe Collas

  • 1Institute of Medical Biochemistry, University of Oslo, Blindern, Oslo, Norway. philippe.collas@basalmed.uio.no

Cloning and Stem Cells
|March 11, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multimodal epigenetic and enhancer network remodeling shape the transcriptional landscape of human beige adipocytes.

Communications biology·2026
Same author

Loss of multilevel 3D genome organization during breast cancer progression.

Genome research·2025
Same author

Transcriptional and Metabolic Changes Following Repeated Fasting and Refeeding of Adipose Stem Cells Highlight Adipose Tissue Resilience.

Nutrients·2025
Same author

Alternative isoform expression of key thermogenic genes in human beige adipocytes.

Frontiers in endocrinology·2024
Same author

Cytoskeletal rearrangement precedes nucleolar remodeling during adipogenesis.

Communications biology·2024
Same author

Loss of multi-level 3D genome organization during breast cancer progression.

bioRxiv : the preprint server for biology·2023
Same journal

Preimplantation development of somatic cell cloned embryos in the common marmoset (Callithrix jacchus).

Cloning and stem cells·2009
Same journal

Identification of multipotent cytotrophoblast cells from human first trimester chorionic villi.

Cloning and stem cells·2009
Same journal

Enhanced generation of myeloid lineages in hematopoietic differentiation from embryonic stem cells by silencing transcriptional repressor Twist-2.

Cloning and stem cells·2009
Same journal

High-efficiency production of subculturable vascular endothelial cells from feeder-free human embryonic stem cells without cell-sorting technique.

Cloning and stem cells·2009
Same journal

Dynamic epigenetic regulation of the Oct4 and Nanog regulatory regions during neural differentiation in rhesus nuclear transfer embryonic stem cells.

Cloning and stem cells·2009
Same journal

Pluripotency and reprogramming: meeting report on the Fifth International Meeting of the Stem Cell Network North Rhine Westphalia.

Cloning and stem cells·2009
See all related articles

Directly reprogramming somatic cells into other cell types via transdifferentiation offers a simpler alternative to nuclear transplantation for therapeutic cell production. This study demonstrates functional cell reprogramming using cellular extracts, paving the way for novel regenerative medicine strategies.

Area of Science:

  • Cell biology
  • Regenerative medicine
  • Epigenetics

Background:

  • Transdifferentiation, directly converting one somatic cell type to another, bypasses challenges in nuclear transplantation.
  • Adult stem cells exhibit broader differentiation potential than previously understood.
  • Novel strategies for transdifferentiation are under active development.

Purpose of the Study:

  • To demonstrate functional reprogramming of somatic cells using cellular extracts.
  • To explore the potential of in vitro cell reprogramming for therapeutic applications.
  • To establish a system for studying nuclear reprogramming mechanisms.

Main Methods:

  • Utilized nuclear and cytoplasmic extracts from T cells to reprogram 293T fibroblasts.
  • Assessed reprogramming by observing nuclear transcription factor assembly, chromatin remodeling, and gene expression changes.

Related Experiment Videos

  • Analyzed the expression of T cell-specific surface molecules and functional characteristics.

    • Main Results:

    • Successfully reprogrammed fibroblasts into cells exhibiting T cell characteristics.
    • Observed nuclear uptake and assembly of transcription factors.
    • Induced chromatin remodeling, altered chromatin composition, and activated lymphoid cell-specific genes.
    • Reprogrammed cells expressed T cell-specific surface markers and demonstrated regulatory function.

    Conclusions:

    • In vitro cell reprogramming using cellular extracts is feasible and functional.
    • This method holds promise for generating isogenic replacement cells for therapeutic purposes.
    • The system provides a valuable tool for investigating the mechanisms of nuclear reprogramming.