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The kinase insert domain-containing receptor (KDR) is regulated by shear stress
T Staalesen1, B Risberg, E Mattsson
1Department of Vascular Surgery, The Wallenberg Laboratory, Bruna Stråket 11B, Plan 1, Sahlgrenska University Hospital, SE-413 45 Gothenburg, Sweden.
Objective:
Intimal hyperplasia develops in areas with low shear stress. The aim of the present study was to investigate if the mRNA expression of vascular endothelial growth factor (VEGF), Fms-like tyrosine kinase-1 receptor (Flt-1) and kinase insert domain-containing receptor (KDR) is regulated by shear stress.
Design:
Endothelial cells from human umbilical veins were in an in vitro system subjected to different levels of shear stress during 1 and 12 h. The mRNA expression of VEGF, Flt-1 and KDR was measured with RT-PCR. eNOS served as positive control and actin as housekeeping gene.
Results:
The KDR expression was isolated upregulated 3-4 times after 12 h exposure to high shear stress.
Conclusion:
The genetic expression of KDR is upregulated by shear stress and this response is time dependent.