Quantitative HPLC analysis of withanolides in Withania somnifera
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Updated: Sep 27, 2026
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M Ganzera1, M I Choudhary, I A Khan
1Department of Pharmacognosy, University of Innsbruck, Innsbruck 6020, Austria. markus.ganzera@uibk.ac.at
Abstract:
One of the most widely used herbs in Ayurvedic medicine is Ashwaghanda, Withania somnifera, a shrub commonly found on the Indian subcontinent. As this plant is increasingly becoming a popular adaptogenic in the western world, analytical methods for its identification and quality control are in demand. Thus, a HPLC method for the determination of withaferin A and withanolide D was developed. The system was successfully used to investigate the presence of the markers in different W. somnifera plant parts as well as to analyze their content in market products.
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