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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
Published on: May 29, 2012
Determination of residual double bonds in resin-dentin interface by Raman spectroscopy
1Department of Operative Dentistry, Nihon University School of Dentistry, 1-8-13, Kanda Surugadai, Chiyoda-Ku, 101-8310, Tokyo, Japan. miyazaki-m@dent.nihon-u.ac.jp
Summary
Researchers used laser Raman spectroscopy to measure unpolymerized adhesive resin in the hybrid layer. This technique quantified residual double bonds, revealing variations between different dental adhesives.
Area of Science:
- Dental Materials Science
- Biomaterials Engineering
- Spectroscopy
Background:
- Hybrid layer quality is crucial for dental restoration longevity.
- Assessing the degree of conversion in the hybrid layer is essential.
- Residual unpolymerized monomers can compromise material properties.
Purpose of the Study:
- To develop and validate a method for quantifying residual double bonds in the dentin-resin interface.
- To utilize laser Raman spectroscopy for analyzing adhesive resin quality.
- To investigate the percentage of unreacted adhesive resin within the hybrid layer.
Main Methods:
- Bovine dentin was bonded with dental adhesives and composite resin.
- Specimens were sectioned and polished to create a smooth interface.
- Laser Raman spectroscopy measured residual double bonds (C=C) relative to ester groups (C=O).
Main Results:
- Residual double bonds ranged from 10-25% across different adhesives.
- Fluoro Bond and Single Bond showed higher percentages of residual double bonds.
- No correlation was found between residual double bond percentage and interface location.
Conclusions:
- Laser Raman microscopy is an effective tool for measuring residual double bonds in the dentin-resin interface.
- The study provides a quantitative method to assess hybrid layer quality.
- Variations in residual monomer content were observed among tested adhesives.
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