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Complementary characteristics of homologous p-octiphenyl beta-barrels with ion channel and esterase activity
Abhigyan Som1, Naomi Sakai, Stefan Matile
1Department of Organic Chemistry, University of Geneva, CH-1211 Geneva 4, Switzerland.
Abstract:
We report that decreasing beta-sheet length in homologous multifunctional rigid-rod beta-barrels with internal histidines increases ion channel stability by three orders of magnitude, reduces binding activity by four orders of magnitude, and reduces esterase activity up to 22-times. These results are further used to evaluate methods employed to characterize suprastructure and activity of synthetic multifunctional pores formed by p-octiphenyl beta-barrels with emphasis on applicability of the Hille model to determine internal diameters and the Woodhull equation to locate internal active sites.
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