Related Experiment Video
Updated: May 7, 2026
Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
Published on: September 26, 2019
Isolation of peptides useful for differential diagnosis of Crohn's disease and ulcerative colitis
H Saito1, Y Fukuda, K Katsuragi
1Department of In Vitro Diagnostics, Otsuka Life Science Initiative, Otsuka Pharmaceutical Co. Ltd, Tokushima, Japan. saitohh@assay.atsuka.co.jp
Background:
Phage displayed random peptide technology has been utilised to identify binding epitopes of antibodies or receptor ligands.
Aim:
To isolates peptides from a phage library which are specifically recognised by antibodies in serum from patients with Crohn's disease (CD).
Methods:
A phage displayed random peptide library composed of nine amino acids was established and sequentially screened using serum immunogloblin G obtained from CD patients.
Results:
Five different CD specific peptides were isolated from the phage library. No homology in amino acid sequences was observed among four (CDP-1, -3 to -5) of the five peptides exhibiting different binding characteristics with each CD patient's serum. In contrast, two peptides (CDP-1 and -2) had similar amino acid sequences and similar binding characteristics. Four multiple antigenic peptides (MAP, CDP-1, -3 to -5) were synthesised, and an enzyme linked immunosorbent assay (ELISA) using the four peptides was developed to detect serum antibodies against them. Fifty two of 92 CD patients (56.5%) were detected by ELISA, none of 20 ulcerative colitis (UC) patients, only one of 25 duodenal ulcer patients, and only three of 48 healthy subjects.
Conclusions:
ELISA using the four peptides isolated in this study may be useful for the differential diagnosis of CD and UC.
