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Updated: Sep 27, 2026

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
Published on: June 20, 2019
A hydrophobic platform as a mechanistically relevant transition state stabilising factor appears to be present in the
W Nerinckx1, T Desmet, M Claeyssens
1Department of Biochemistry, Physiology and Microbiology, Ghent University, K L Ledeganckstraat 35, B-9000 Gent, Belgium. wim.nerinckx@rug.ac.be
Abstract:
An in silico survey of the -1 subsite of all known 3D-structures of O-glycoside hydrolases containing a suitably positioned ligand has led to the recognition -- apparently without exceptions -- of a transition state stabilising hydrophobic platform which is complementary to a crucial hydrophobic patch of the ligand. This platform is family-specific and highly conserved. A comprehensive list is given with examples of enzymes belonging to 33 different families. Several typical constellations of platform - protein residues are described.
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