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Specific conjugation of DNA binding proteins to DNA templates through thiol-disulfide exchange
Valeri G Metelev1, Elena A Kubareva, Olga V Vorob'eva
1Chemical Department, M V Lomonosov Moscow State University, Vorob'evi gori, Moscow 119992, Russia.
FEBS Letters
|March 14, 2003
Abstract:
The double-stranded oligodeoxyribonucleotides with single internucleotide disulfide linkages were successfully used for covalent trapping of cysteine containing protein. In particular, an efficient conjugation of DNA methyltransferase SsoII to sequence-specific decoys was demonstrated. The obtained results assume that synthetic oligodeoxyribonucleotides bearing a new trapping site can be used as new tools to study and manipulate biological systems.