Slowing of rat diaphragm action potential depolarization by endurance treadmill training

This study tested the hypothesis that the action potential properties of the diaphragm muscle are altered by endurance exercise treadmill training. Rats underwent treadmill running or sham training for 8 weeks, and intracellular electrophysiological recordings were subsequently performed in vitro. Diaphragm resting membrane potential was not altered by training. The maximal rate of action potential depolarization was reduced significantly by exercise training, from 551+/-16 to 445+/-15 mV/ms (P<0.00002). In contrast the rate of action potential repolarization was not significantly different between the two groups (P=0.25). Action potential height was significantly higher in control compared with trained muscle (84.5+/-1.0 vs. 78.4+/-1.2 mV, P<0.0005). The combination of slowed action depolarization and decreased peak action potential height resulted in no net change in action potential area. Thus treadmill running endurance exercise training slows rat diaphragm action potential depolarization but not repolarization, suggestive of altered Na+ but not K+ channel function.