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Serum free and phospholipid-bound choline decrease after surgery and methylprednisolone administration in dogs.

Yesim Ozarda Ilcol1, Zeki Yilmaz, Ismail H Ulus

  • 1Department of Biochemistry, Merkez Laboratory, Uludag University Medical School, 16059 Gorukle, Bursa, Turkey. yesim@uludag.edu.tr

Neuroscience Letters
|March 14, 2003
PubMed
Summary

Surgery and methylprednisolone treatment decrease serum choline levels in dogs, linked to elevated cortisol. This study reveals glucocorticoids as the cause of reduced choline post-surgery.

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Area of Science:

  • Biochemistry
  • Veterinary Medicine
  • Endocrinology

Background:

  • Serum choline is vital for various physiological processes.
  • Glucocorticoids play a role in metabolic regulation.
  • The impact of surgical stress and corticosteroid treatment on choline levels requires further investigation.

Purpose of the Study:

  • To investigate the effects of surgery and methylprednisolone on serum free and phospholipid-bound choline concentrations in dogs and rats.
  • To explore the relationship between serum cortisol levels and choline concentrations following surgery.
  • To elucidate the role of glucocorticoids in mediating changes in choline levels.

Main Methods:

  • Serum samples were collected from dogs and rats before and after abdominal-pelvic surgery.

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  • Choline concentrations (free and phospholipid-bound) and cortisol levels were measured.
  • Methylprednisolone was administered to dogs at varying doses (5-20 mg/kg).
  • Statistical analysis, including correlation analysis, was performed.

    • Main Results:

    • In dogs, surgery led to significant decreases in both free (29%) and phospholipid-bound (17%) choline, persisting for 24 hours.
    • Post-surgery cortisol elevation in dogs was inversely correlated with choline reductions (r=-0.737 for free choline, r=-0.771 for bound choline).
    • Methylprednisolone administration in dogs caused dose- and time-dependent decreases in choline levels.
    • In rats, surgery decreased phospholipid-bound choline but did not affect free choline; methylprednisolone had no significant effect on free choline.

    Conclusions:

    • Surgical stress and methylprednisolone treatment significantly reduce serum choline concentrations in dogs.
    • Elevated circulating glucocorticoids, such as cortisol, are identified as the primary cause for the decrease in serum choline observed after surgery.
    • These findings highlight the impact of the endocrine stress response on choline homeostasis.