Spike frequency adaptation and signaling properties of identified neurons in rodent deep spinal dorsal horn

Using whole cell recordings, I analyzed the intrinsic discharge properties for 285 neurons in Rexed's laminae III-V of isolated hamster spinal cord preparations. Neurons were characterized by their responses to step-wise and ramp-hold depolarizing current applied through the recording pipettes. Tonic cells (133/285; 47%) fired repetitively during step-wise current application. Firing decayed linearly (-0.14 to -4.3 imp . s(-1) . s(-1)) or was bimodal, with an initial exponential phase (tau approximately 450 ms) followed by a linear decline (-0.02 to -6.3 imp . s(-1) . s(-1)); discharge frequency was unrelated to current trajectory. Phasic-firing cells (108/285; 38%) responded with a burst discharge having an initial rapid, exponential decrease (tau approximately 30 ms) and subsequent linear decline (-1 to -78 imp . s(-1) . s(-1)). Phasic cells were activated preferentially by fast current ramps (slope, 70 pA/s-2.2 nA/s) with the number and frequency of impulses increasing with current slope. Delayed-firing cells (44/285; 15%), responded to current steps with an accelerating firing following a substantial latent period (0.5-4 s) and discharged during current ramps with slopes less than approximately 100 pA/s. Intracellular staining revealed a significant association between electrophysiological profile and neuronal morphology. A majority of presumed projection cells (22/30; 73%) exhibited tonic firing to step-wise activation. The preponderance of phasic and delayed firing cells, 93% (42/45) and 71% (12/17), respectively, were interneurons with local or intersegmental terminations. Differential sensitivity to static and time-varying components of membrane current suggest differences in neuronal signaling properties that may have important implications for integration of mechanosensory information in the deep spinal dorsal horn.