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Published on: April 13, 2012
Marker assisted detection of gene (1Dx5) and translocation (1B/1R) in wheat genotypes
Ratan Tiwari1, Priyamvada, Rajender Singh
1Directorate of Wheat Research, P.O. Box 158, Karnal 132 001, India. tiwari 1964@yahoo.com
Abstract:
Detection of 1Dx5 gene and presence of 1B/1R wheat rye translocation were studied in nineteen elite Indian wheat genotypes using AS-PCR and STS markers, respectively. Fifteen genotypes had 1B/1R translocation whereas ten showed presence of 1Dx5 gene. More than 50 per cent of the genotypes tested were found positive for both 1Dx5 and 1B/1R translocation. The results are in conformity with HMW glutenin SDS-PAGE profile for 1Dx5 and cytological observations for 1B/1R translocation.
